Open AccessPhytomitogen‐induced, lymphokine‐mediated cartilage proteoglycan degradation
Author(s) -
Herman Jerome H.,
Musgrave Diane S.,
Dennis Marie V.
Publication year - 1977
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780200404
Subject(s) - proteoglycan , lymphokine , cartilage , heterologous , immunology , in vitro , chemistry , matrix (chemical analysis) , microbiology and biotechnology , biology , biochemistry , anatomy , chromatography , gene
The significance of T‐cell activation in the immunopathogenesis of articular disease has been studied in an in vitro model to assess the capacity of phytomitogen‐induced lymphokines to degrade cartilage matrix proteoglycans. Supernatants derived from T‐cell activation of normal human peripheral blood lymphocytes were shown to contain a monocyte‐dependent, serum‐inhibitable, dialyzable factor or factors capable of inducing significant proteoglycan degradation, as assessed by release of macroprecipitable 35 S‐labeled proteoglycan from heterologous cartilage substrates.