Open AccessInteractions of extractable nuclear antigen (ENA) and double stranded DNA
Author(s) -
Hamburger Max,
Friedlander Lester,
Barland Peter
Publication year - 1974
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780170418
Subject(s) - dna , nuclear dna , incubation , microbiology and biotechnology , chemistry , antigen , biology , biophysics , biochemistry , genetics , gene , mitochondrial dna
Extractable nuclear antigen (ENA) has been shown to inhibit the combination of anti‐DNA with double stranded DNA. ENA has also been found to complex with DNA by two different techniques. In one, red cells coated with DNA were shown to retain significantly more radiolabeled ENA than uncoated controls. In a second, DNA‐ 125 I was found to be retained on a membrane filter after incubation with ENA, indicating DNA‐protein complex formation. It is suggested that the ENA may complex with DNA in vivo and that these complexes may retard the formation of anti‐DNA‐DNA complexes.