Open AccessLysosome stabilization by adjuvant arthritis serum
Author(s) -
Persellin Robert H.
Publication year - 1972
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780150203
Subject(s) - acid phosphatase , lysosome , adjuvant , arthritis , enzyme , chemistry , endocrinology , medicine , alkaline phosphatase , rheumatoid arthritis , immunology , biochemistry , pharmacology
Evidence is presented that serum from rats with adjuvant arthritis stabilizes the membranes of isolated normal rat liver lysosomes. The stabilizer retarded the release of the lysosomal enzymes, acid phosphatase and β‐glucuronidase, but not the release of the mitochondrial enzyme, isocitric dehydrogenase. Stabilizing activity in serum paralleled adjuvant disease, being greatest when the inflammation was most intense and subsiding as the arthritis diminished. Activity could be removed from the serum by absorption with isolated membranes; adjuvant arthritis serum did not inhibit enzyme activities. The membrane reactant was neither a corticosteroid nor an antibody but was a large molecular weight substance with charge properties similar to an α‐globulin.