Open AccessThe detection of anti‐globulin factors utilizing pre‐coated latex particles
Author(s) -
Singer Jacques M.,
Plotz Charles M.,
Goldberg Rosalie
Publication year - 1965
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780080203
Subject(s) - gamma globulin , latex fixation test , agglutination (biology) , titer , globulin , chromatography , materials science , chemistry , immunology , antibody , medicine
A modified serological procedure for the detection of anti‐gamma globulin factors has been developed. This test employs polystyrene latex particles with a diameter of 0.25 microns. The particles are coated with gamma globulin and the excess protein in solution is removed by repeated washing. A solution of these gamma globulin coated particles is stable for at least one year, and yields more reproducible titers than those originally described for the latex agglutination test.