Open AccessStudies of the rabbit antibodies which sensitize red blood cells for agglutination by rheumatoid factors
Author(s) -
Rockey John H.,
Kunkel Henry G.
Publication year - 1961
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.1780040502
Subject(s) - antibody , agglutination (biology) , titer , red blood cell , rheumatoid factor , ultracentrifuge , rabbit (cipher) , red cell , chemistry , microbiology and biotechnology , immunology , antibody titer , sensitization , cell , biology , biochemistry , medicine , mathematics , statistics
The high and low molecular weight antibodies of rabbit antisheep red cell serum were separated by electrophoresis, density gradient ultracentrifugation, and DEAE chromatography. It was demonstrated that only antibodies of the low molecular weight class were able to sensitize cells in subagglutinating titer for agglutination by rheumatoid factors. High molecular weight antibodies lacked this capacity. The roles of incomplete rabbit antirabbit red cell and antisheep red cell antibodies in sensitization is discussed.