Open AccessA role for chemokines in the induction of chondrocyte phenotype modulation
Author(s) -
Mazzetti Ilaria,
Magagnoli Giorgia,
Paoletti Samantha,
Uguccioni Mariagrazia,
Olivotto Eleonora,
Vitellozzi Roberta,
Cattini Luca,
Facchini Andrea,
Borzì Rosa Maria
Publication year - 2004
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.11474
Subject(s) - chemokine receptor , microbiology and biotechnology , chondrocyte , cxcr3 , cxc chemokine receptors , chemokine , ccl17 , biology , chemistry , receptor , cartilage , biochemistry , anatomy
Objective To extend the study of the chemokine receptor repertoire on human chondrocytes to receptors with reported housekeeping functions (CXCR3, CXCR4, CXCR5, and CCR6) and to evaluate whether ligands of these receptors play a role in chondrocyte phenotype modulation and proliferation. Methods Chemokine receptor expression was determined by flow cytometry. Subcultures of chondrocytes were collected and fixed at confluence or during the exponential phase of growth and analyzed for chemokine receptor modulation. The effects of chemokines on isolated cells as well as chondrocytes cultured within an intact extracellular matrix were investigated. Isolated human chondrocytes were stimulated with 100 n M chemokines (monokine induced by interferon‐γ, stromal cell–derived factor 1α [SDF‐1α], B cell–attracting chemokine 1 [BCA‐1], or macrophage inflammatory protein 3α), and conditioned media were assessed for matrix‐degrading enzyme contents (matrix metalloproteinases [MMPs] 1, 3, and 13, and N ‐acetyl‐β‐ D ‐glucosaminidase [NAG]). Cell proliferation and phenotype modulation were evaluated by bromodeoxyuridine incorporation and cathepsin B production. Induction of cell proliferation was assessed in cartilage explants by immunodetection of the proliferation‐associated antigen S100A4. Results CXCR3, CXCR4, CXCR5, and CCR6 were detected on human chondrocytes. CXCR3 and CXCR4 expression was increased in exponentially growing chondrocyte subcultures. Ligands of all receptors enhanced the release of MMPs 1, 3, and 13. Release of NAG and cathepsin B was significantly higher in chemokine‐stimulated cultures than in unstimulated cultures. SDF‐1α and BCA‐1 also induced DNA synthesis and chondrocyte proliferation, as was shown by the up‐regulation of S100A4 in cartilage explants as well. Conclusion Our findings extend the repertoire of functional responses elicited by the activity of chemokines on chondrocytes and open new avenues in our understanding of the control of chondrocyte differentiation status by chemokines and their receptors.