Open AccessA novel polymorphism in the Fcγ receptor IIB (CD32B) transmembrane region alters receptor signaling
Author(s) -
Li Xiaoli,
Wu Jianming,
Carter Robert H.,
Edberg Jeffrey C.,
Su Kaihong,
Cooper Glinda S.,
Kimberly Robert P.
Publication year - 2003
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.11313
Subject(s) - nonsynonymous substitution , biology , transmembrane domain , microbiology and biotechnology , b cell receptor , genetics , receptor , b cell , antibody , gene , genome
Abstract Objective The low‐affinity receptor Fcγ receptor IIb (FcγRIIb), with an immunoreceptor tyrosine‐based inhibitory motif (ITIM) in the cytoplasmic domain, down‐regulates humoral immune responses and modulates the risk of autoimmunity in animal models. The transmembrane domain of FcγRIIb may also contribute to receptor signaling. Therefore, we investigated the biologic significance of single‐nucleotide polymorphisms (SNPs) throughout the coding region. Methods Discovery of SNPs in FCGR2B was performed by direct cycle sequencing of complementary DNA samples derived by reverse transcriptase–polymerase chain reaction. To assess the biologic significance of the nonsynonymous transmembrane SNP, we studied 3 functions influenced by the FcγRIIb transmembrane domain: tyrosine dephosphorylation of CD19, inhibition of B cell receptor (BCR)–induced calcium response, and modulation of BCR‐ or anti‐Fas–induced apoptosis. Results The nonsynonymous C‐to‐T transition in the first cytoplasmic exon, originally reported in the Raji cell line, was not found in either the African‐American or the Caucasian population, but a nonsynonymous T‐to‐C transition at nucleotide 775 in exon 4 of FCGR2B , which changes isoleucine to threonine at residue 187 in the transmembrane domain, was significantly more common in African Americans. Using the FcγRIIb‐negative mouse B cell line IIA1.6, we expressed both allelic forms as both full‐length and truncated cytoplasmic domain constructs. The FCGR2B ‐187T allele mediated a higher level of CD19 dephosphorylation ( P = 0.029) and a greater degree of inhibition of the calcium response ( P = 0.003) when co‐engaged with BCR than did FCGR2B ‐187I, independent of the presence of the ITIM. In contrast, FcγRIIb modulation of BCR‐induced and anti‐Fas antibody–induced cell death rates were similar in IIA1.6 cells expressing either the 187I or the 187T allelic form. Conclusion The differential activity of FCGR2B alleles suggests a novel mechanism of FcγRIIb regulation that may influence the risk of autoimmune disease.