Open AccessReduction of CpG‐induced arthritis by suppressive oligodeoxynucleotides
Author(s) -
Zeuner Rainald A.,
Ishii Ken J.,
Lizak Martin J.,
Gursel Ihsan,
Yamada Hiroshi,
Klinman Dennis M.,
Verthelyi Daniela
Publication year - 2002
Publication title -
arthritis & rheumatism
Language(s) - English
Resource type - Journals
eISSN - 1529-0131
pISSN - 0004-3591
DOI - 10.1002/art.10423
Subject(s) - cpg oligodeoxynucleotide , arthritis , inflammation , medicine , immune system , hyperplasia , immunology , cpg site , peripheral blood mononuclear cell , pathology , chemistry , biochemistry , gene expression , dna methylation , in vitro , gene
Objective Bacterial DNA contains immunostimulatory CpG motifs that cause inflammation when injected into the knee joints of normal mice. We examined whether synthetic oligodeoxynucleotides (ODN) that suppress CpG‐induced immune responses prevent CpG‐induced arthritis. Methods CpG, suppressive, and/or control ODN were injected into the knees of BALB/c mice. Joint swelling and inflammation were evaluated by physical measurement, by histologic analysis of joint tissue, and by magnetic resonance imaging. Results Immunostimulatory CpG DNA induced local arthritis, characterized by swelling of the knee joints, the presence of inflammatory cell infiltrates, the perivascular accumulation of mononuclear cells, and hyperplasia of the synovial lining. Administering suppressive (but not control) ODN reduced the manifestations and severity of arthritis up to 80%. Conclusion Suppressive ODN may be useful for the prevention or treatment of arthritis induced by bacterial DNA.