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Protective effect of corynoline in a murine allergic rhinitis model via inhibition of caspase‐1/NF‐κB
Author(s) -
Wei Li,
Ren Dan,
Zhao Guna,
Zhao Lin
Publication year - 2021
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.202000231
Subject(s) - ovalbumin , histamine , chemistry , western blot , pharmacology , docking (animal) , nf κb , apoptosis , immunology , biochemistry , medicine , immune system , gene , nursing
Allergic rhinitis (AR) is a serious public health concern worldwide. Therefore, the present study was conducted to scrutinize the protective effect of corynoline (COR) against ovalbumin (OVA)‐induced AR in BALB/c mice. The effect of COR was investigated on various parameters, such as nose‐rub score, histamine intensity, level of cytokines, and NF‐κB binding activity. It was found that COR causes a significant reduction in the nose‐rub score with a reduction in histamine intensity. It also causes reductions in cytokines, such as TNF‐α, IL‐1β, and MIP‐2, in comparison to OVA‐challenged mice. COR reduces the gene expression of active caspase‐1 in Western blot analysis, together with inhibition of NF‐κB binding activity. The inhibitory effect on NF‐κB binding was further substantiated by docking analysis, where COR excellently docked into the active site of NF‐κB via the creation of H‐bond and π–cation interactions with Lys145. Taken altogether, our results demonstrated that COR could be used as a potential therapeutic agent against AR.