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Synthesis, characterization, and biological studies of chalcone derivatives containing Schiff bases: Synthetic derivatives for the treatment of epilepsy and Alzheimer's disease
Author(s) -
Koçyiğit Ümit M.,
Gezegen Hayreddin,
Taslimi Parham
Publication year - 2020
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.202000202
Subject(s) - chalcone , chemistry , acetylcholinesterase , carbonic anhydrase , schiff base , stereochemistry , sulfanilamide , chemical synthesis , affinity chromatography , carbonic anhydrase i , enzyme , in vitro , biochemistry
In this study, first, Schiff base‐containing chalcone derivatives were synthesized. The human carbonic anhydrase (hCA) isoenzymes I and II were then purified from human erythrocytes using Sepharose‐4B‐ l ‐tyrosine‐sulfanilamide affinity chromatography. In addition, the inhibitory effects of the newly synthesized compounds on the activities of hCA and acetylcholinesterase (AChE) were investigated in vitro, using the esterase and acetylcholine iodide method. The IC 50 values were determined and the K i values of AChE and hCA activities were calculated from the Lineweaver–Burk graphs determined in this study. The hCA I isoform was inhibited by these chalcone derivatives containing Schiff bases ( 3a–j and 5a–f ) in low nanomolar levels, whose K i values ranged between 141.88 ± 24.10 and 2,234.47 ± 38.11 nM. Against the physiologically dominant isoform hCA II, the compounds demonstrated K i values varying from 199.31 ± 40.45 to 602.79 ± 263.22 nM. Also, these compounds effectively inhibited AChE, with K i values ranging from 20.41 ± 6.04 to 125.94 ± 23.88 nM. According to these results, the newly synthesized molecules were found to be potent inhibitors of these enzymes.