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Synthesis, Cytotoxicity by Bioluminescence Inhibition, Antibacterial and Antifungal Activity of ([1,2,4]Triazolo[1,5‐ c ]quinazolin‐2‐ylthio)carboxylic Acid Amides
Author(s) -
Antipenko Lyudmila N.,
Karpenko Alexander V.,
Kovalenko Sergey I.,
Katsev Andrew M.,
KomarovskaPorokhnyavets Elena Z.,
Novikov Vladimir P.
Publication year - 2009
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.200900077
Subject(s) - cytotoxicity , antimicrobial , chemistry , bioluminescence , aspergillus niger , antibacterial activity , stereochemistry , biological activity , escherichia coli , thiadiazoles , aspergillus flavus , bacteria , combinatorial chemistry , biochemistry , organic chemistry , in vitro , biology , food science , gene , genetics
We report in this work the synthesis, cytotoxicity, and antimicrobial activity of ([1,2,4]triazolo[1,5‐ c ]quinazolin‐2‐ylthio)carboxylic acid amides 4 – 7 in connection with our previous research in the preparation of triazoloquinazoline derivatives. Due to simplicity, general availability of starting materials, and high yields, the most reliable method of synthesis appeared to be the one with N , N ‐carbonyldiimidazole activation stage. The chemical structures of all obtained substances were deduced from FT‐IR, 1 H‐NMR, EI‐MS, and LC‐MS spectral data. The results of cytotoxicity evaluated by bioluminescence inhibition of bacterium Photobacterium leiognathi, strain Sh1 showed that compounds 4.1 , 4.6 , and 6.1 were the most cytotoxic. Investigation of the antimicrobial and antifungal activity of amides 4 – 7 (concentration 5 mg/mL) was carried out by the stiff‐plate agar‐diffusion method. We found that the compounds possessed low ( 4.1 , 4.7 ) antifungal activity against Candida tenuis and strong ( 4.21 , 5.1 , 5.9 ) or inefficient ( 4.7 , 4.12 , 4.16 ) activity against Aspergillus niger . Substances 5.1 and 5.9 slightly affected Mycobacterium luteum . Staphylococcus aureus was resistant to all obtained substances, and only the n ‐butyramide derivatives 7.1 and 7.5 inhibited the growth of Escherichia coli. Hence, there was no strong correlation between bioluminescence inhibition and antimicrobial activity of the investigated substances.