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New Lanthanum (III) Complex – Synthesis, Characterization, and Cytotoxic Activity
Author(s) -
Kostova Irena,
Rastogi Vinod K.,
Kiefer Wolfgang,
Kostovski Aleksandar
Publication year - 2006
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.200600077
Subject(s) - chemistry , lanthanum , ligand (biochemistry) , aqueous solution , lanthanide , inorganic chemistry , cytotoxic t cell , stereochemistry , organic chemistry , biochemistry , in vitro , ion , receptor
The complex of lanthanum (III) was synthesized reacting the respective inorganic salt with 5‐aminoorotic acid in amounts equal to the metal : ligand molar ratio of 1 : 3. The complex was prepared by adding an aqueous solution of lanthanum (III) nitrate to an aqueous solution of the ligand, subsequently raising the pH of the mixture gradually to approx. 5.0 through addition of a dilute solution of sodium hydroxide. The structure of the final complex was determined by means of spectral data (IR, Raman, 1 H‐NMR) and elemental analysis. Significant differences in the IR spectrum of the complex were observed as compared to the spectrum of the ligand. A comparative analysis of the Raman spectrum of the complex with that of the free 5‐aminoorotic acid allowed a straightforward assignment of the vibrations of the ligand groups involved in coordination. The ligand and the complex were tested for the cytotoxic activities on the chronic myeloid leukemia derived K‐562, overexpressing the BCR‐ABL fusion protein and the non‐Hodgkin lymphoma derived DOHH‐2, characterized by a re‐expression of the anti‐apoptotic protein bcl‐2 cell lines. The results obtained indicate that the tested compounds exerted a considerable cytotoxic activity upon the evaluated cell lines in a concentration‐dependent matter, which enabled the construction of dose‐response curves and the calculation of the corresponding IC 50 values. The inorganic salt exerted a very weak cytotoxic effect on these cells, which is in contrast to the lanthanum (III) complex.