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Study on the Decomposition of 2‐Chloroethylnitro‐ sosulfamides (CENS) in Serum Using HPLC On‐line Solid Phase Extraction
Author(s) -
Seridi Achour,
Winum JeanYves,
Kadri Mekki,
Abdaoui Mohamed,
Montero JeanLouis
Publication year - 2006
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.200600071
Subject(s) - chemistry , high performance liquid chromatography , decomposition , chromatography , partition coefficient , aqueous solution , extraction (chemistry) , kinetics , aqueous two phase system , solid phase extraction , phase (matter) , organic chemistry , physics , quantum mechanics
In this paper we have investigated the kinetic decomposition of 2‐chloroethylnitrososulfamides (CENS) in fetal calf serum. The study was monitored by on‐line solid phase extraction linked to RP‐LC‐MS. We demonstrated that CENS are less stable in fetal calf serum than in aqueous buffer at pH 7.4 and 37°C. Moreover, we have shown that partition coefficient of CENS can be correlated to the kinetics decomposition, and we observe that the stability is better for lipophilic CENS. The different metabolites after decomposition have been tentatively identified by RP‐HPLC‐MS. This study indicates the formation of several metabolites resulting from a mechanism of decomposition more complex than in aqueous phosphate buffer, nevertheless, leading to the same main products.