Premium
Determination of Formoterol by Capillary Electrophoresis and its Application to Inhaler Capsules
Author(s) -
Şener Erol,
Tunçel Muzaffer,
AboulEnein Hassan Y.
Publication year - 2003
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.200300733
Subject(s) - detection limit , capillary electrophoresis , formoterol , chromatography , inhaler , chemistry , electrophoresis , capillary action , dry powder inhaler , acetonitrile , analytical chemistry (journal) , materials science , medicine , inhalation , asthma , anesthesia , composite material , budesonide
A capillary electrophoretic (CE) method for the determination of formoterol (FOR) in a pharmaceutical preparation is described. Analysis was made in a background electrolyte consisting of 20 % acetonitrile and 50 mMphosphoric acid at pH 2.5, using fused silica capillary (86 cm x 75 μm ID), 27 kV potential, and detecting at 200 nm. Under these electrophoretic conditions 3, 4‐dihydroxybenzylamine used as an internal standard (IS) and FOR showed symmetrical peaks at 6.1 and 8.3 min., respectively. The inter‐day and intra‐day precision was examined in the concentration range of 2.98 ? 10 ‐6 M to 8.94 x 10 ‐6 M. Good correlation and accuracy were obtained. Limit of detection, (LOD) and limit of quantitation (LOQ) values were 3.71 x 10 ‐7 M and 1.11 x 10 ‐6 M, respectively. The method was applied for the analysis of FOR in pharmaceutical inhaler capsules. The proposed method is reliable, precise, accurate, fast, and cost effective.