Phosphono Analogues of Glutathione as New Inhibitors of Glutathione S‐Transferases

Phosphono‐analogues of glutathione containing the O=P(OR) 2 moiety in place of the cysteinyl residue CH 2 SH 1a–1d were prepared by solution phase peptide synthesis. Benzyl, benzyloxycarbonyl, and tert ‐butyl protecting groups were used to mask the individual amino acid functional groups. The formation of peptide bonds was achieved by the usual peptide synthesis via activation of carboxylic functions with cyclohexylcarbodiimide and subsequent reaction with free amino groups. The thus obtained, fully‐protected peptides were each purified by normal phase column chromatography. Deprotection was accomplished by hydrogenolysis and by treatment with HBr/acetic acid yielding the desired phosphonic acid diester 1a–1d . The inhibition of the glutathione conjugation of 1‐chloro‐2,4‐dinitrobenzene by human placental glutathione S‐transferase was studied by determining the IC 50 values of the new glutathione analogues. The IC 50 values were 291 μM, 139 μM, 64 μM, and 21 μM for the dimethyl, diethyl, diisopropyl, and di‐ n ‐butyl esters, respectively. The results clearly show that the formal substitution of the glutathione thiol function by phosphonic acid esters leads to a new class of glutathione S‐transferase inhibitors. Further investigations directed at the question of whether or not these glutathione analogues are suitable for a modulation in chemotherapy are in progress.