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Microsomal N ‐Oxygenation of Adenine to Adenine 1‐ N ‐Oxide
Author(s) -
Clement Bernd,
Kunze Thomas
Publication year - 1993
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.19933260106
Subject(s) - chemistry , microsome , phenobarbital , metabolite , cytochrome , enzyme , high performance liquid chromatography , chromatography , biochemistry , pharmacology , medicine
During investigations on the N ‐oxygenation of adenine ( 1 ) the enzymatic formation of adenine 1‐ N ‐oxide 3 was demonstrated for the first time. The identity of this metabolite was confirmed by its chromatographic behaviour and UV‐spectrum recorded after HPLC separation. Adenine 1‐ N ‐oxide ( 3 ) and similar oxygenated derivatives of adenine were synthesized as reference substances. The enzymatic formation of 3 exhibits the typical characteristics of a reaction catalysed by microsomal mono‐oxygenases. In induction experiments, an increase in the rate of formation of 3 after pretreatment with phenobarbital was observed. A participation of those isoenzymes of the cytochrome P‐450 enzyme system which can be induced by phenobarbital is assumed.