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Rapid Assay for the Quantification of Piretanide in Biological Fluids
Author(s) -
SpahnLangguth Hildegard,
Hahn Gabriele,
Mutschler Ernst
Publication year - 1991
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.19913240708
Subject(s) - chromatography , bumetanide , chemistry , urine , elution , high performance liquid chromatography , extraction (chemistry) , diuretic , pharmacology , biochemistry , membrane , medicine , ion transporter
Piretanide was determined from plasma and urine using reversed‐phase high‐performance liquid chromatography. Plasma was extracted with diethylether at pH 4 using bumetanide as internal standard. The chromatographic separation was performed on an octadecylsilane column (ODS) with acetonitrile/pH 7 phosphate buffer (3:7, v/v). In order to determine piretanide in urine, bumetanide was added to a urine aliquot. Then the sample was centrifuged and the supernatant directly injected without extraction followed by chromatography on an ODS column with a mixture of methanol and pH 7 phosphate buffer (12:13, v/v). The compounds were detected by their intrinsic fluorescence, monitoring the eluate at 287/450 nm. Total chromatography times were 8 min for plasma and 13 min for urine samples. The method was applied for the assay of piretanide in plasma of healthy volunteers after i.v. or p.o. dosage of 6 mg piretanide.