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Determination of Some β‐Blockers as Dabsyl Derivatives by HPLC
Author(s) -
Alpertunga Buket,
Sungur Sidika,
Ersoy Lale,
Manav Sabiha Yalçin
Publication year - 1990
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.19903230906
Subject(s) - oxprenolol , pindolol , acebutolol , chemistry , chromatography , metoprolol , high performance liquid chromatography , propranolol , methanol , organic chemistry , medicine , biochemistry , receptor
A HPLC method has been developed that permits the sensitive determination of beta adrenergic blocking drugs, including acebutolol, metoprolol, oxprenolol, pindolol, and propranolol. These compounds were converted to their chromophoric dabsyl derivatives and were separated by a reversed phase chromatographic column (μ.‐Bondapak C 18 ) with methanol‐water (75:25) as isocratic mobile phase. The derivatives were detected by a variable wavelength dedector operating at 430 nm. The method was applied to commercial pharmaceutical preparations and the results were statistically compared with those obtained by official methods using t‐ and F‐tests.