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Quantitative Determination of Brofaromin in Human Plasma after High Performance Liquid Chromatographic Separation
Author(s) -
Horne C.,
Mutschler E.
Publication year - 1988
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.19883210613
Subject(s) - chromatography , chemistry , detection limit , high performance liquid chromatography , pharmacokinetics , extraction (chemistry) , quantitative analysis (chemistry) , human plasma , liquid–liquid extraction , heptane , pharmacology , organic chemistry , medicine
The quantitative determination of a new antidepressant, the MAO‐inhibiting agent brofaromine, in human biological material is described. Analysis is performed by alkaline extraction with n‐heptane‐isopropanol (99:1, v/v), subsequent fluorescence derivatisation with NBD chloride (4‐chloro‐7‐nitrobenzofurazan), high performance liquid chromatography, and fluorimetric measurement of the derivatisation product (λ max ex. = 465 n, λ max , em. = 515 nm). The sensitivity of the method (detection limit < 1 ng/ml) is suitable for pharmacokinetic studies after application of therapeutic doses of brofaromine.