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Formation of Active Oxygen Species by Dithranol, III Dithranol, Active Oxygen Species and Lipid Peroxidation in vivo
Author(s) -
Müller Klaus,
Wiegrebe Wolfgang,
Younes Maged
Publication year - 1987
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/ardp.19873200110
Subject(s) - dithranol , chemistry , lipid peroxidation , formazan , in vivo , superoxide , oxygen , theaflavin , superoxide dismutase , cytochrome c , biochemistry , photochemistry , enzyme , nuclear chemistry , antioxidant , organic chemistry , biology , mitochondrion , immunology , psoriasis , microbiology and biotechnology , polyphenol
Abstract At pH 7.8 the dithranol anion 1a is oxidized in the dark to yield the bianthrone 3 besides dithranol brown, whilst O 2 is reduced to O 2 ·− and H 2 O 2 . O 2 ·− was detected by the reduction of nitroblue‐tetrazolium (NBT, 4 ). The formation of the formazan 5 was inhibited by superoxide dismutase (SOD). Exclusion of O 2 and addition of SOD reveal a direct electron transfer from 1a to 4 and to cytochrome c which was not affected in the presence of SOD. — In vivo experiments indicate a low rate of lipid peroxidation which was determined on the basis of ethane exhalation.