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Induction of diaphorase‐1 by dicoumarol in Drosophila virilis larvae
Author(s) -
Georgieva T. G.,
Jankulova E. D.,
Ralchev K. H.,
Dunkov B. C.
Publication year - 1995
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940290104
Subject(s) - dicoumarol , drosophila virilis , biology , in vitro , in vivo , larva , drosophila (subgenus) , immunoprecipitation , biochemistry , microbiology and biotechnology , enzyme , nad+ kinase , genetics , botany , gene
Drosophila diaphorase‐1 (DIA‐1) is an enzyme similar to mammalian DT‐diaphorase and is inhibitied in vitro by dicoumarol. However, a ten‐fold increase in DIA‐1 activity was observed when third instar Drosophila virilis larvae were fed on a diet containing 0.1 M dicoumarol for 48 h. This induction was shown to be dose dependent and immunoprecipitation experiments with DIA‐1 antiserum demonstrated an increase in the DIA‐1 protein level in dicoumarol‐treated larvae. The induction of DIA1 by dicoumarol was found to be blocked by actinomycin D, which suggests a transcriptional mechanism of regulation. The opposite effect of dicoumarol on DIA‐1 in vitro vs. in vivo suggests that a metabolic conversion takes place after the ingestion of this compound by D. virilis larvae. © 1995 Wiley‐Liss, Inc.