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Fate of a terminal olefin with Drosophila microsomes and its inhibitory effects on some P‐450 dependent activities
Author(s) -
Cuany André,
Helvig Christian,
Amichot Marcel,
Pflieger Philippe,
Mioskowski Charles,
Salaun JeanPierre,
Pauron David,
Larroque Christian,
Berge JeanBaptiste
Publication year - 1995
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940280403
Subject(s) - microsome , biology , biochemistry , metabolism , bioassay , lauric acid , incubation , enzyme , strain (injury) , fatty acid , genetics , anatomy
In vitro bioassays were used to analyze the metabolism of the 11‐dodecenoic acid (11‐DDNA) by microsomes prepared from Drosophila melanogaster RalDDT R strain. 11‐DDNA is metabolized to 11,12‐epoxylauric acid (epoxyLA) in a NADPH‐dependent way. The microsomal production of epoxyLA reaches a plateau very quickly, suggesting the occurrence of an enzyme inactivation process. After incubation of microsomes with (1‐ 14 C)11‐DDNA, three proteins of Mr ≈ 50 kDa were labeled. 11‐DDNA inhibits the microsomal metabolism of lauric acid and 7‐ethoxycoumarin in a time and NADPH‐dependent process. An inhibition of metabolites generated from DDT and testosterone was also obtained but at higher concentrations. These results are discussed according to the fact that RalDDT R is an insecticide resistant strain characterized as a high metabolizer of the insecticide DDT and also of lauric acid, testosterone, and ethoxycoumarin. © 1995 Wiley‐Liss, Inc.
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