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Inhibition by azadirachtin of phospholipid transfer from lipophorin to the oocytes in Rhodnius prolixus
Author(s) -
Moreira Monica F.,
Garcia Eloi S.,
Masuda Hatisaburo
Publication year - 1994
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940270405
Subject(s) - hemolymph , rhodnius prolixus , phospholipid , biology , yolk , vitellogenesis , azadirachtin , endocrinology , medicine , biochemistry , oocyte , insect , botany , microbiology and biotechnology , membrane , embryo , food science , pesticide , agronomy
The growth of oocytes was severely reduced in female Rhodnius prolixus treated with azadirachtin A (AZA). When administered in a blood meal, AZA (1 μg/ml) inhibited phospholipid transfer to the ovaries without altering the availability of yolk protein in the hemolymph or its uptake by 1 mm oocytes. The lipid composition of lipophorin, its concentration in the hemolymph, and its density were normal in AZA‐treated females. Partial inhibition of phospholipid transfer was observed when lipophorin from AZA‐treated females was injected into normal females, or when lipophorin from normal females was injected into AZA‐treated females. The two effects were additive, so that the transfer of phospholipids from the lipophorin of AZA‐treated females to the oocytes of AZA‐treated females was nearly eliminated. In combination, these effects of AZA on phospholipid transfer to the oocytes limit the capacity of AZA‐treated females to produce eggs. © 1994 Wiley‐Liss, Inc.