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Cytochrome b 5 involvement in cytochrome P450 monooxygenase activities in house fly microsomes
Author(s) -
Zhang Minli,
Scott Jeffrey G.
Publication year - 1994
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940270306
Subject(s) - cytochrome b5 , cytochrome , monooxygenase , cytochrome p450 , microsome , biochemistry , antiserum , biology , cytochrome p450 reductase , reductase , cytochrome b , cytochrome c , enzyme , coenzyme q – cytochrome c reductase , gene , genetics , mitochondrion , antibody , mitochondrial dna
The involvement of cytochrome b 5 in different cytochrome P450 monooxygenase and palmitoyl CoA desaturase activities in microsomes from insecticide‐resistant (LPR) house flies was determined using a specific polyclonal antiserum developed against house fly cytochrome b 5 . Anti‐b 5 antiserum inhibited the reduction of cytochrome b 5 by NADH‐cytochrome b 5 reductase. The antiserum also inhibited palmitoyl CoA desaturase, methoxycoumarin‐ O ‐demethylase (MCOD), ethoxycoumarin‐ O ‐deethylase (ECOD), and benzo[ a ]pyrene hydroxylase (aromatic hydrocarbon hydroxylase, AHH) activities. However, methoxyresorufin‐ O ‐demethylase (MROD) and ethoxyresorufin‐ O ‐deethy‐lase (EROD) activities were not affected by this antiserum. These results demonstrate that cytochrome b 5 is involved in fatty acyl CoA desaturase activities and in certain cytochrome P450 monooxygenase activities (i.e., MCOD, ECOD, and AHH) in LPR house fly microsomes. Other cytochrome P450 monooxygenase activities (i.e., MROD and EROD) may not require cytochrome b 5 . The results suggest that cytochrome b 5 involvement with cytochrome P450 monooxygenase activities is dependent upon the cytochrome P450 isoform involved. © 1994 Wiley‐Liss, Inc.