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Storage protein uptake in helicoverpa zea : Arylphorin and VHDL share a single receptor
Author(s) -
Wang Zhixiang,
Haunerland Norbert H.
Publication year - 1994
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940260103
Subject(s) - biology , endocytosis , receptor , biochemistry , helicoverpa zea , storage protein , fat body , microbiology and biotechnology , gene , botany , larva , noctuidae
The major lepidopteran storage protein arylphorin is selectively taken up into perivisceral fat body of prepupae by receptor mediated endocytosis. The arylphorin receptor was identified by ligand blotting and in vitro binding studies. Fat body membranes contain a glycosylated receptor protein with an apparent molecular weight of 80,000 that binds arylphorin with a K D of 9.02 × 10 −8 M. Competitive binding experiments revealed that the arylphorin receptor is identical with the previously identified VHDL receptor. Apparently a single receptor mediates the uptake of structurally distinct storage proteins. This storage protein receptor is present only in perivisceral fat body and only during the period around pupation when both storage proteins are sequestered. © 1994 Wiley‐Liss, Inc.