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Age and tissue‐dependent changes of ornithine decarboxylase and s‐adenosylmethionine decarboxylase activities in neural tissue and fat body of adult crickets
Author(s) -
Cayre Myriam,
Tirard Alain,
Charpin Pierre,
Augier Roger,
Strambi Colette,
Strambi Alain
Publication year - 1993
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940240405
Subject(s) - ornithine decarboxylase , acheta , biology , enzyme , endocrinology , medicine , nervous tissue , biochemistry , metabolism , spermidine , adenosylmethionine decarboxylase , methionine , enzyme assay , developmental profile , central nervous system , biosynthesis , amino acid , cricket , ecology , neuroscience
The activities of ornithine decarboxylase and S‐adenosylmethionine decarboxylase, two key enzymes in polyamine metabolism, were determined during the first 10 days of imaginal life in the nervous tissue and the fat body of the adult cricket Acheta domesticus. The kinetic constants of the two enzymes were also determined in both tissues. Both decarboxylases presented a higher activity in fat body than in nervous tissue. In nervous tissue, the activity of the two enzymes peaked at 16 h postemergence, then slowly decreased up to day 3–4. By contrast, the enzymatic activities in fat body, low at emergence, strongly increased on day 2. Thereafter, whereas ornithine decarboxylase activity remained rather high. S‐adenosyl‐methionine decarboxylase activity dropped back to emergence levels by day 10. These results, examined in light of the temporal alterations of polyamine levels observed in the two tissues, demonstrate synchronous variations between polyamine contents and the enzymes involved in their biosynthesis. © 1993 Wiley‐Liss, Inc.