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Neutral sterols and ecdysteroids of the solitary cactus bee Diadasia rinconis cockerell (hymenoptera: Anthophoridae)
Author(s) -
Feldlaufer Mark F.,
Lusby William R.,
Weirich Gunter F.,
Svoboda James A.,
Buchmann Stephen L.
Publication year - 1993
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940230205
Subject(s) - ecdysteroid , biology , sterol , hymenoptera , radioimmunoassay , botany , biochemistry , cholesterol , larva
Using high performance liquid chromatography in conjunction with radioimmunoassay and mass spectrometry, the major ecdysteroid of the solitary cactus bee, Diadasia rinconis , was determined to be 20‐hydroxyecdysone, with lesser amounts of makisterone A. Another 28‐carbon ecdysteroid thought to be the 24‐epimer of makisterone A was also detected. The neutral sterols of Diadasia consisted primarily of 24‐methylenecholesterol (92.2%) with lesser amounts of other C 28 and C 29 sterols. Cholesterol accounted for less than 0.1% of the total tissue sterols. The occurrence of 20‐hydroxyecdysone in a phytophagous hymenopteran is discussed in relation to the low level of cholesterol encountered. © 1993 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.