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Insecticidal potential of the insect parvovirus Gm DNV
Author(s) -
Tal Jacov,
Attathom Tipvadee
Publication year - 1993
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940220304
Subject(s) - biology , galleria mellonella , instar , larva , chloramphenicol acetyltransferase , reporter gene , dna , gene , virology , spodoptera , insect , pyralidae , gene expression , microbiology and biotechnology , botany , genetics , recombinant dna , virulence
The insecticidal potential of Galleria mellonella densovirus ( Gm DNV) in third, fourth, and fifth instar larvae of the host, the greater wax moth, as a step toward the construction of a molecular vector for the introduction and expression of foreign genes in the larvae of these insects was studied. Third instar larvae are most susceptible to Gm DNV. Viral RNA synthesis is more rapid in this stage and slowest in the fifth instar. Infection of prepupae by intradermic injection or by horzontal spread inhibited pupation. Gm DNV DNA is also infectious when introduced as a calcium phosphate precipitate. The two putative viral promoters were shown to be capable of driving the expression of the reporter gene chloramphenicol acetyltransferase (CAT) in DNA‐injected larvae. © 1993 Wiley‐Liss, Inc.