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Characteristics of Locusta migratoria diuretic hormone
Author(s) -
Lehmberg Elisabeth,
Schooley David A.,
Ferenz HansJ.,
Applebaum Shalom W.
Publication year - 1993
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940220111
Subject(s) - malpighian tubule system , corpus allatum , hemolymph , biology , endocrinology , medicine , secretion , hormone , diuretic , excretory system , juvenile hormone , amino acid , biochemistry , midgut , larva , botany
Locusta migratoria diuretic hormone (Lom‐DH) is a linear 46 amino acid peptide, amidated at the C‐terminus. The purified native hormone and synthetic peptide similarly stimulate the secretion of cAMP by isolated Malpighian tubules in vitro in a dose‐dependent manner. The degree of stimulated fluid secretion in the isolated Malpighian tubule assay is similar for the native and synthetic hormone. Based on ELISA assay, Lom‐DH is mainly found in the storage lobes of the corpora cardiaca (∼ 12 pmol per gland pair) and to a small extent in the brain. Marginal amounts can be detected in the hemolymph. No detectable amounts were found in the glandular lobes of the corpora cardiaca or in the corpora allata or subesophageal ganglion. © 1993 Wiley‐Liss, Inc.