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Acetylcholine receptors of thoracic dorsal midline neurones in the cockroach, Periplaneta americana
Author(s) -
Bai Donglin,
Erdbrugger Heinrich,
Breer Heinz,
Sattelle David B.
Publication year - 1992
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940210406
Subject(s) - periplaneta , oxotremorine , mecamylamine , pirenzepine , acetylcholine , cockroach , bicuculline , muscarinic acetylcholine receptor , biology , atropine , cholinergic , nicotinic agonist , acetylcholine receptor , endocrinology , medicine , anatomy , receptor , gabaa receptor , biochemistry , ecology
The actions of acetylcholine and cholinergic ligands have been studied using dorsal midline neurones from the metathoracic ganglion of the cockroach Periplaneta americana . Both nicotine and oxotremorine depolarized dorsal midline neuronal cell bodies. Dose‐response curves for nicotine and oxotremorine saturated at different levels. Nicotine‐induced depolarizations were completely or partially blocked by mecamylamine, d‐tubocurarine, strychnine, and bicuculline, but were insensitive to α‐bungarotoxin (100 nM), atropine (100 μM), scopolamine (10 μM), and pirenzepine (50 μM). Following pretreatment with collagenase, the dorsal midline neurones were sensitive to high doses of α‐bungarotoxin (3 μM). Oxotremorine‐induced depolarizations were blocked by scopolamine (10 μM), atropine (100 μM), and pirenzepine (50 μM) and were insentive to mecamylamine (10 μM) and d‐tubocurarine (100 μM). The results indicate the coexistence of at least two distinct acetylcholine receptors on dorsal midline neuronal cell bodies in the cockroach metathoracic ganglion. © 1992 Wiley‐Liss, Inc.