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Identification of yolk platelet‐associated hydrolases in the oocytes of Rhodnius prolixus
Author(s) -
Nussenzveig Roberto H.,
Oliveira Pedro L.,
Masuda Hatisaburo
Publication year - 1992
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940210402
Subject(s) - rhodnius prolixus , pepstatin , acid phosphatase , biochemistry , biology , yolk , platelet , enzyme , cathepsin d , plasmin , cathepsin , alkaline phosphatase , microbiology and biotechnology , insect , immunology , protease , ecology
The yolk platelets from Rhodnius prolixus , a blood‐sucking bug, are composed mostly of vitellin and here are shown to contain at least two hydrolytic enzymes, a phosphatase and a cathepsin D‐like proteinase. Both the proteinase and the phosphatase have an acid pH optimum. No hydrolytic activity was observed under alkaline or neutral conditions. Among several proteinase inhibitors tested, only pepstatin could abolish vitellin breakdown in vitro. The proteinase appears to be bound to the yolk platelet membranes. The phosphatase activity, using p ‐nitrophenyl phosphate as substrate, was enhanced after disruption of the platelet membrane by Triton X‐100. This activity could be inhibited by tartrate but not by p ‐cloromercuribenzoate. © 1992 Wiley‐Liss, Inc.