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Separation of insect hemolymph proteins by cascade‐mode multiaffinity chromatography
Author(s) -
Samaraweera Preminda,
Law John H.,
Porath Jerker
Publication year - 1992
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940200402
Subject(s) - hemolymph , manduca sexta , biology , fractionation , chromatography , insect , column chromatography , biochemistry , chemistry , botany
The hemolymph of the adult female Manduca sexta was fractionated by cascade‐mode multiaffinity chromatography (CASMAC) on a main‐line tandem column chain containing Zn 2+ ‐TED, T‐gel, Ni 2+ ‐DPA, and phenylsepharose and a side‐line column containing Zn 2+ ‐DPA. The technique separated some of the previously described major hemolymph proteins, and yielded a number of fractions with simple composition. Some of these fractions contained only less abundant proteins of Manduca hemolymph. Thus, it appears that CASMAC would be a very useful fractionation technique for purification and characterization of the minor proteins of insect hemolymph. © 1992 Wiley‐Liss, Inc.