Separation of insect hemolymph proteins by cascade‐mode multiaffinity chromatography | Zendy

Samaraweera Preminda | Zendy; Law John H. | Zendy; Porath Jerker | Zendy

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Separation of insect hemolymph proteins by cascade‐mode multiaffinity chromatography

Author(s) -

Samaraweera Preminda,

Law John H.,

Porath Jerker

Publication year - 1992

Publication title -

archives of insect biochemistry and physiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.576

H-Index - 66

eISSN - 1520-6327

pISSN - 0739-4462

DOI - 10.1002/arch.940200402

Subject(s) - hemolymph , manduca sexta , biology , fractionation , chromatography , insect , column chromatography , biochemistry , chemistry , botany

The hemolymph of the adult female Manduca sexta was fractionated by cascade‐mode multiaffinity chromatography (CASMAC) on a main‐line tandem column chain containing Zn 2+ ‐TED, T‐gel, Ni 2+ ‐DPA, and phenylsepharose and a side‐line column containing Zn 2+ ‐DPA. The technique separated some of the previously described major hemolymph proteins, and yielded a number of fractions with simple composition. Some of these fractions contained only less abundant proteins of Manduca hemolymph. Thus, it appears that CASMAC would be a very useful fractionation technique for purification and characterization of the minor proteins of insect hemolymph. © 1992 Wiley‐Liss, Inc.

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