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A FMRFamide‐like peptide is associated with the myotropic ovulation hormone in Rhodnius prolixus
Author(s) -
Sevala Veeresh L.,
Sevala Venkatalakshmi M.,
Davey Kenneth G.,
Loughton Barry G.
Publication year - 1992
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940200304
Subject(s) - fmrfamide , hemolymph , rhodnius prolixus , biology , antiserum , endocrinology , medicine , neuropeptide , ovulation , titer , peptide , hormone , antibody , insect , immunology , biochemistry , receptor , botany
The protocerebral neurosecretory cells previously shown to be the source of the myotropin controlling ovulation in Rhodnius prolixus react in an immunocytochemical assay using an antiserum against FMRFamide. When the same antiserum was injected into fed mated females at the appropriate time the timing of oviposition was delayed, but the total number of eggs developed was unaffected compared to controls injected with pre‐immune rabbit serum. The titer of FMRFamide‐like peptide (assayed by RIA) in the hemolymph of mated and virgin females was found to fluctuate with the egg laying cycle, and to reflect earlier determinations of the titer of myotropic activity. Western blots of SDS‐PAGE revealed a FMRFamide‐immunoreactive peptide of approximately 8.5 kDa in both hemolymph and extracts of the ovulation hormone cells. © 1992 Wiley‐Liss, Inc.