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Allatotropin‐Induced formation of inositol phosphates in the corpora allata of the moth, Manduca sexta
Author(s) -
Reagan Jeff D.,
Miller Wilma H.,
Kramer Steven J.
Publication year - 1992
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940200206
Subject(s) - manduca sexta , corpus allatum , biology , juvenile hormone , inositol phosphate , inositol , medicine , endocrinology , sphingidae , second messenger system , biochemistry , biosynthesis , manduca , intracellular , insect , enzyme , hormone , receptor , botany
Allatotropin (AT) is a 13 amino acid neuropeptide isolated from the tobacco hornworm, Manduca sexta , which stimulates the biosynthesis of juvenile hormone (JH) in adult females of this insect. In this paper we report that AT stimulates inositol phosphate formation in both female and male corpora allata (CA) of adult Manduca sexta . Furthermore, thapsigargin, a potent Ca 2+ ‐ATPase inhibitor, staurosporine, a potent inhibitor of protein kinases A and C, and 2,5‐di‐(tert‐butyl)‐1,4‐hydroquinone (DBHQ), a potent inhibitor of liver microsomal ATP‐dependent Ca 2+ sequestration, all stimulate JH biosynthesis without changing the basal level of inositol phosphates. These data suggest that AT activates an inositol 1,4,5‐trisphosphate (IP 3 ) pathway. Furthermore, increases in the free intracellular Ca 2+ concentration in the CA stimulate JH biosynthesis. © 1992 Wiley‐Liss, Inc.