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Makisterone C: A 29‐carbon ecdysteroid from developing embryos of the cotton stainer bug, Dysdercus fasciatus
Author(s) -
Feldlaufer Mark F.,
Weirich Gunter F.,
Lusby William R.,
Svoboda James A.
Publication year - 1991
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940180202
Subject(s) - ecdysteroid , biology , ecdysone , sterol , embryo , titer , insect , sphingidae , campesterol , botany , endocrinology , manduca sexta , larva , cholesterol , microbiology and biotechnology , genetics , antibody
An ecdysteroid RIA was used to determine the ecdysteroid titer in developing embryos of Dysdercus fasciatus and revealed that peak titer occurred approximately 120 h post‐oviposition. Analysis of neutral sterols at this time indicated sitosterol to be the predominant neutral sterol with lesser amounts of campesterol. Embryonic sterols were highly reflective of the sterols found in the cotton seed diet upon which previous generations of the bugs had fed. Analysis of the embryonic extract for ecdysteroids indicated the presence of both makisterone A and the 29‐carbon ecdysteroid makisterone C. Isolation of these compounds was accomplished by reversed‐phase and silica HPLC in conjunction with RIA, and the identification of both compounds was confirmed by mass spectrometry. No ecdysone or 20‐hydroxyecdysone was detected in the embryonic sample.