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Purification of ribosomal subunits from Ceratitis capitata and comparison of their proteins from two different developmental stages
Author(s) -
Pilali Maria M.,
Fragoulis Emmanuel G.
Publication year - 1991
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940160107
Subject(s) - ceratitis capitata , biology , ribosomal rna , ribosome , ribosomal protein , protein subunit , capitata , eukaryotic large ribosomal subunit , biochemistry , eukaryotic small ribosomal subunit , rna , microbiology and biotechnology , botany , gene , tephritidae , pest analysis , brassica oleracea
Ribosomal subunits have been prepared from larvae of Ceratitis capitata at two different developmental stages. The subunits are stable and pure on the basis of recentrifugation experiments and characterization of the RNA contained in them. When ribosomal subunits were allowed to combine, they reassociated to form an 80S ribosome, which is active in poly(U)‐directed polyphenylalanine synthesis. The proteins derived from these subunits were characterized by one and two dimensional electrophoresis and the patterns obtained from 6‐day old larvae and white prepupae were compared.