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PCR‐generated conspecific sodium channel gene probe for the house fly
Author(s) -
Knipple Douglas C.,
Payne Lori L.,
Soderlund David M.
Publication year - 1991
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940160106
Subject(s) - biology , drosophila melanogaster , gene , housefly , genomic dna , genetics , polymerase chain reaction , melanogaster , homology (biology) , sodium channel , musca , sequence analysis , oligonucleotide , microbiology and biotechnology , sodium , botany , chemistry , organic chemistry , larva
A segment of the house fly (Musca domestica) homologue of the para (paralytic) sodium channel gene of Drosophila melanogaster was isolated by using mixed sequence oligonucleotide primers in the polymerase chain reaction (PCR). The specificity of the procedure was demonstrated by genomic Southern analysis using the housefly PCR amplification product as a probe and by DNA sequence analysis. The latter showed structural homology to the para gene, but not to the corresponding region of DSC1 , another D. melanogaster gene with structural similarity to vertebrate sodium channel genes.