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On the action of RH 5849, a nonsteroidal ecdysteroid agonist, on a cell line from Chironomus tentans
Author(s) -
SpindlerBarth Margarethe,
Turberg Andreas,
Spindler KlausDieter
Publication year - 1991
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940160103
Subject(s) - ecdysteroid , biology , ecdysone , medicine , endocrinology , agonist , receptor , biochemistry , hormone
RH 5849 competes with 3 H‐ponasterone A for ecdysteroid binding sites in Chironomus tentans cells with an about fourfold lower relative affinity as compared to 20‐OH‐ecydsone. It does not interfere with glucocorticoid and estrogen binding sites in vertebrates. It is also not recognized by an ecdysteroid antibody. RH 5849 exerts typical morphological and physiological effects ascribed to the action of 20‐OH‐ecdysone on C. tentans cells, namely an increase in acetylcholinesterase activity and an inhibition of chitin synthesis.