Premium
Biological effects of synthetic AKH in Manduca sexta and estimates of the amount of AKH in corpora cardiaca
Author(s) -
Ziegler Rolf
Publication year - 1990
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940150206
Subject(s) - manduca sexta , adipokinetic hormone , sphingidae , glycogen phosphorylase , biology , endocrinology , medicine , manduca , glycogen , biochemistry , fat body , larva , botany , gene
Dose‐response curves were measured with synthetic Manduca adipokinetic hormone (AKH) for glycogen phosphorylase activation in larvae and for lipid mobilization in adults. Both responses are known hormonal functions in Manduca sexta . In ligated larvae, full activation of glycogen phosphorylase was achieved with 0.1 pmol and half‐maximal activation with 0.03–0.04 pmol. Maximal lipid mobilization in adults required 10 pmol and half‐maximal mobilization 0.15 to 0.2 pmol, respectively. An estimate of AKH content of corpora cardiaca from M. sexta was gained by comparing the dose‐response curves for synthetic Manduca AKH with curves from gland extracts. Corpora cardiaca extracts were also quantitated by high performance liquid chromatography. According to both estimates corpora cardiaca of adults contain 10–20 pmol AKH per pair, while a pair of larval corpora cardiaca contains 0.7–2 pmol.