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Sex‐linked control of sex pheromone behavioral responses in European corn‐borer moths ( Ostrinia nubilalis ) confirmed with TPI marker gene
Author(s) -
Glover Thomas,
Campbell Marlene,
Robbins Paul,
Roelofs Wendell
Publication year - 1990
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940150202
Subject(s) - ostrinia , european corn borer , biology , sex pheromone , hybrid , pheromone , locus (genetics) , lepidoptera genitalia , pyralidae , genetics , botany , gene , zoology
In two races of European corn‐borer moths (ECB), the E‐race females emit and males respond to 99:1 sex pheromone blend of ( E )/( Z )‐11‐tetradecenyl acetates, whereas the Z‐race females and males produce and respond to the opposite 3:97 pheromone blend of ( E )/( Z )‐11‐tetradecenyl acetates, respectively. We previously have shown that female production of the final blend ratio is under control of a major autosomal locus but that the sequence of male upwind flight responses to the blend is controlled by a sex‐linked (Z‐linked) locus. This sex‐linked control of behavioral responses in crosses of E and Z ECB now is confirmed by use of sex‐linked TPI (triose phosphate isomerase) allozyme phenotypes to determine the origin of the sex chromosomes in F 2 populations. F 1 males from reciprocal E × Z crosses generate similar behavioral‐response profiles in wind‐tunnel studies, with moderate numbers responding to the Z pheromone and intermediate blends (35%–65% Z), but very few responding to the E pheromone. The F 2 behavioral‐response profiles indicate that they are composed of 1:1 mixtures of hybrids and paternal profiles. Analysis of TPI allozyme differences allowed us to separate male F 2 populations into individuals whose Z chromosomes both originated from their grandfathers, and individuals who had one Z chromosome originating from each grandparent. With these partitioned F 2 s, the TPI homozygotes exhibited behavioral‐response profiles very much like their grandfathers, whereas the TPI hybrids produced response profiles similar to their heterozygous F 1 fathers. These results demonstrate incontrovertibly that the response to sex pheromone in male ECB is controlled by a sex‐linked gene that is tightly linked to the TPI locus and therefore is independent of the locus controlling pheromone blend production in females.