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P1 gene expression in Drosophila larval fat body: Induction by various Ecdysteroids
Author(s) -
SomméMartin Ghislaine,
Colardeau Jacqueline,
Beydon Philippe,
Blais Catherine,
Lepesant Jean Antoine,
Lafont René
Publication year - 1990
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940150105
Subject(s) - biology , ecdysteroid , 20 hydroxyecdysone , ecdysone , mutant , gene expression , gene , fat body , transcription (linguistics) , drosophila (subgenus) , microbiology and biotechnology , larva , drosophila melanogaster , transcription factor , biochemistry , botany , linguistics , philosophy
Using the developmental mutant ecd1, the biological activity of 20‐hydroxyecdysone (20E) and 20E metabolites 3‐dehydro‐20‐hydroxyecdysone (3D20E), 3‐epi‐20‐hydroxyecdysone (3D20E), 3‐epi‐20‐hydroxyecdysone‐3‐phosphate (20E′3P), 20,26‐dihydroxyecdysone (20,26E), and 20‐hydroxyecdysonoic acid (20Eoic) was tested for their ability to induce the transcription of the steroid‐inducible gene P1 in the Drosophila larval fat body. 3D20E was the most efficient ecdysteroid in the initiation of P1 gene transcription therefore the formation of 3D20E and the 3‐epimer could not be regarded as an inactivation pathway in Drosophila larvae. Formation of 20,26E and 20Eoic may be an inactivation pathway in this biological model.