Premium
In vitro study of lipid metabolism in the mealworm larval fat body
Author(s) -
Bahjou Ahmed,
Gourdoux Lucienne,
Moreau Robert,
Puiroux Jacques,
Dutrieu Janine
Publication year - 1990
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940150103
Subject(s) - mealworm , adipokinetic hormone , trehalose , lipogenesis , hemolymph , biology , lipid metabolism , fat body , in vitro , biochemistry , metabolism , larva , medicine , botany , gene
Lipid metabolism in Tenebrio larval fat body has been studied in vitro. Lipid release required the presence of diluted hemolymph in the incubation medium. This time‐dependent release of lipid was strongly stimulated in a dose‐dependent manner by Tenebrio corpora cardiaca (CC) extracts or synthetic adipokinetic hormone (AKH I). Furthermore, some glycerol was released when larval fat body was incubated without hemolymph, and this phenomenon was also dose dependent for added CC extracts. Lipid synthesis was estimated in vitro by following the incorporation of radioactivity from [6‐ 14 C] glucose into fatty acids. Lipogenesis occurred in the absence of added carbohydrates in the medium, but it was stimulated by the addition of glucose, and especially trehalose (10 mg ml −1 ). Intestinal insulin‐like peptide (ILP) also stimulated in vitro lipogenesis in a dose‐dependent fashion. We conclude that lipolytic and lipogenetic activities of larval mealworm fat body in vitro are effectively under hormonal control.