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High‐affinity uptake of [ 3 H]serotonin in cultured neurones of the cockroach Periplaneta americana
Author(s) -
Bermudez Isabel,
Beadle David J.
Publication year - 1989
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940120405
Subject(s) - veratridine , periplaneta , serotonin , biology , picrotoxin , deltamethrin , cockroach , pargyline , biochemistry , pharmacology , biophysics , chemistry , sodium , sodium channel , antagonist , receptor , organic chemistry , pesticide , agronomy , ecology
Abstract Cultured central neurons from the American cockroach, Periplaneta americana , have been used to investigate the uptake of [ 3 H]serotonin. The neurones accumulate [ 3 H]serotonin from the extracellular medium by both a high‐and a low‐affinity system. The activity of the high‐affinity mechanism is decreased by low temperature and metabolic poisons, and is dependent on sodium and chloride ions. Both depolarising levels of external potassium ions and veratridine decrease the high‐affinity uptake system, suggesting it is influenced by the transmembrane potential. The pyrethroid insecticides, deltamethrin and permethrin, enhance the inhibitory effect of veratridine. Pyrethroid enhancement is completely blocked by tetrodotoxin, and neither pyrethroid affects the uptake system in the absence of veratridine. Avermectin B 1A is a powerful inhibitor of the high‐affinity uptake system, and its effect is blocked by picrotoxin. High‐affinity uptake of [ 3 H]serotonin is inhibited by imipramine and amitriptyline; desipramine has no significant effect on this uptake. The activity of the high‐affinity system is also reduced by 8‐hydroxy‐dipropylaminotetralin, α‐methyl‐serotonin, and 1‐(3‐chlorophenyl)piperazine. Dopamine, noradrenaline, octopamine, and the formamidine insecticides, chlordimeform and demethylchlordimerform, are moderate inhibitors of the high‐affinity uptake system. The formamidine effect is not blocked by tetrodotoxin or picrotoxin.