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Juvenile hormone identification, titer, and degradation during the last larval stadium of Spodoptera littoralis
Author(s) -
Zimowska Grazyna,
Rembold Heinz,
Bayer Gabrielle
Publication year - 1989
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940120102
Subject(s) - hemolymph , spodoptera littoralis , juvenile hormone , biology , titer , noctuidae , stadium , fat body , degradation (telecommunications) , esterase , lepidoptera genitalia , larva , biochemistry , botany , enzyme , antibody , immunology , telecommunications , geometry , mathematics , computer science
Juvnile hormone (JH) was identified and titers determined during the last larval stadium of the cotton leafworm Spodoptera littoralis using combined gas chromatography‐selected ion monitoring‐mass spectrometry. JH was almost exclusively limited to JH II, occurring at two major peaks: the first at the beginning of the stadium and the second at days 4 and 5 just after wandering. Degradation of JH and α‐naphthyl acetate (α‐NA) in hemolymph and fat body was determined during the same developmental periods. JH degradation in hemolymph occurred initially as a broad bimodal peak during the second half of feeding, with peaks at days 2 and 3. The second peak occurred at the end of the stadium, subsequent to the second JH II titer peak. Three α‐NA esterase peaks in hemolymph were detected when JH degradation was at a very low level. Fat body JH degradation was resolved as four activity peaks throughout the stadium, with the first three peaks occurring just previous to the JH degradation peaks in the hemolymph. The highest levels of JH and α‐NA degradation activities in fat body were related to the wandering stage.