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Lipid composition of housefly microsomes
Author(s) -
Spates G. E.,
Elissalde M.,
Williams H.
Publication year - 1988
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940070106
Subject(s) - housefly , lysophosphatidylethanolamine , microsome , biochemistry , phospholipid , fatty acid , phosphatidylethanolamine , biology , palmitoleic acid , caproic acid , linoleic acid , phosphatidylcholine , food science , musca , enzyme , botany , membrane , larva
The lipid composition of microsomes isolated from whole‐body preparations of a diazinon‐resistant strain of the housefly ( Musca domestica L.) was determined. When calculated on a mg% basis housefly microsomes are composed of 47% neutral lipids and 53% phospholipids. The free fatty acids compose the major group of compounds among neutral lipids (60%) and the major phospholipid is phosphatidylethanolamine (47.6%). The molar cholesterol/phospholipid ratio is 0.14, ca. twofold higher than the ratio found in mammalian liver microsomes. Fatty acid composition of housefly microsomes is notable only in that there is no uniform distribution of fatty acid moieties throughout the various classes of lipids, except for linoleic acid (18:2), which accounted for 9.2‐12.6% of the neutral lipids. Eighty percent of the fatty acid moieties of phosphatidylserine (+ phosphatidylinositol) were unsaturated. The highest percentage of saturated fatty acid moieties (64.26%) was found in lysophosphatidylethanolamine. Palmitoleic acid (16:1), the major fatty acid found in higher dipterans, ranged from 13.51% in lysophosphatidylethanolamine to 37.45% in the free fatty acids. One prostaglandin (PGF 1d ) and leukotriene B 4 were detected in the microsomal lipids at concentrations of 59.7 and 716 pg/200 mg of protein, respectively.