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Single‐channel recordings of chloride currents in primary cultured Drosophila neurons
Author(s) -
Yamamoto Daisuke,
Suzuki Nobuyuki
Publication year - 1987
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940060303
Subject(s) - chloride channel , chloride , biophysics , patch clamp , biology , current (fluid) , intracellular , ion channel , conductance , electrophysiology , anatomy , biochemistry , chemistry , neuroscience , physics , receptor , organic chemistry , condensed matter physics , thermodynamics
Single‐chloride‐channel currents were recorded from primary cultured Drosophila neurons by means of the gigaohm‐seal patch‐clamp technique. Small inward‐going current channels were observed in excised inside‐out patches with the external face of the membrane exposed to bathing solutions devoid of K + , Na + , and Ca 2+ . The inward current was affected by changing the anions but not the cations bathing the cytoplasmic face of the patch. Complete replacement of CI − by glutamate eliminated the current. The current was maintained with intracellular solutions containing NO 3 − in place of CI − . The single‐channel conductance was estimated to be 7 ps with CI − , and 11 ps with NO 3 − at 10°C. Possible functions of this anion‐selective channel have been discussed.