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Subcellular location of phosphoproteins in salivary glands of the lone star tick, Amblyomma americanum (L.)
Author(s) -
McSwain Janis L.,
Schmidt Stephen P.,
Claypool Deborah M.,
Essenberg Richard C.,
Sauer John R.
Publication year - 1987
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940050104
Subject(s) - amblyomma americanum , biology , tick , salivary gland , fractionation , phosphate , biochemistry , cell fractionation , microbiology and biotechnology , membrane , ixodidae , chromatography , chemistry , ecology
Phosphoproteins were examined by electrophoresis and autoradiography in fractions of tick salivary glands. When whole salivary glands were preincubated in 32 P i , then stimulated by 10 μM dopamine and subsequently fractionated, substantial phosphate was incorporated into 45,000‐, 47,000‐, and 62,000‐dalton proteins of the plasma membrane‐rich 11,500g pellet and 100,000g supernatant. When tissue homogenates were incubated in [γ‐ 32 P] ATP prior to subcellular fractionation, the 62,000‐, 47,000‐, and 45,000‐dalton proteins were enhanced by cyclic AMP in all fractions and were most prominent in the membrane‐rich 11,500g fraction. Phosphoproteins of the same molecular masses were also found in the 11,500g pellet and 100,000g supernatant when labelled with [γ‐ 32 P] ATP in the presence of cAMP.