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Developmental and ecdysteroid regulation of gene expression in the larval fat body of Drosophila melanogaster
Author(s) -
Lepesant JeanAntoine,
Maschat Florence,
KejzlarováLepesant Jana,
Beneš Helen,
Yanicostas Constantin
Publication year - 1986
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940030714
Subject(s) - biology , ecdysteroid , drosophila melanogaster , gene , melanogaster , northern blot , gene expression , transcription (linguistics) , messenger rna , genetics , microbiology and biotechnology , larva , botany , philosophy , linguistics
Six major transcripts encoding the α, β, and γ subunits of the larval serum protein LSP‐1, the single subunit of the LSP‐2, and the P1 and P6 polypeptides appear sequentially in the fat body tissue during the third larval stage of Drosophila melanogaster. Northern blot analysis shows that the transcription of the LSP‐2 and P1 mRNAs is restricted temporally to the third instar and the early phase of pupal development and spatially to the fat body tissue. The synthesis and/or accumulation of the LSP‐2 and P1 transcripts are differentially affected by mutations of the dor and occ loci of the 2B5 complex of genes involved in the trans ‐regulation of the activity of ecdysteroid‐responsive genes. Application of the P element‐mediated germ line transformation to the P1 gene shows that correct developmental expression of this gene requires no more than 1.5 kb of DNA sequences upstream from the mRNA start site.