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Activation of storage protein receptor by 20‐hydroxyecdysone
Author(s) -
Natori Shunji
Publication year - 1986
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940030712
Subject(s) - biology , 20 hydroxyecdysone , receptor , moulting , fat body , microbiology and biotechnology , ecdysone receptor , biochemistry , larva , nuclear receptor , hormone , gene , botany , transcription factor
A storage protein receptor was shown to be present in membranes of the pupal fat body of Sarcophaga peregrina , whereas membranes of the larval fat body contained a cryptic receptor. The molting hormone, 20‐hydroxyecdysone, was essential for activation of the cryptic receptor to bind and incorporate the storage protein. The active receptor in the pupal fat body membrane was a protein with a molecular mass of 120‐kdaltons. It was found that a 125‐kdalton protein, a cryptic receptor for storage protein, present in the larval fat body membrane was converted to an active receptor in the presence of 20‐hydroxyecdysone, without accompanying appreciable new protein synthesis.