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[N‐Methyl‐ 3 H]Scopolamine binding sites in the central nervous system of the cockroach Periplaneta americana
Author(s) -
Lummis Sarah C. R.,
Sattelle David B.
Publication year - 1986
Publication title -
archives of insect biochemistry and physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.576
H-Index - 66
eISSN - 1520-6327
pISSN - 0739-4462
DOI - 10.1002/arch.940030403
Subject(s) - periplaneta , muscarinic acetylcholine receptor , cockroach , nicotinic agonist , acetylcholinesterase , american cockroach , biology , acetylcholine , acetylcholine receptor , cholinergic , ganglion , binding site , central nervous system , nervous system , biochemistry , biophysics , receptor , stereochemistry , anatomy , chemistry , endocrinology , neuroscience , enzyme , ecology
The binding of [N‐methyl‐ 3 H]scopolamine to a cockroach nerve cord preparation has been investigated. Specific [N‐methyl‐ 3 H]scopolamine binding was found to be saturable and of high affinity (K d = 13.9 nM). Muscarinic ligands were found to displace [N‐methyl‐ 3 H]scopolamine binding more effectively than nicotinic ligands. The distribution of these [N‐methyl‐ 3 H]scopolamine binding sites was examined in the metathoracic ganglion at the light microscope level by autoradiographical techniques. Specific binding was found to be localized to distinct regions of the neuropile. This pattern showed certain similarities to that seen when the ganglion was stained for acetylcholinesterase, suggesting a functional role for these insect muscarinic acetylcholine receptors.